FOB Series
Introduction
Fiber-optic evanescent wave biosensors FOB Series are constructed
based on the principle of evanescent wave while light propagates in an
optical fiber. When the target biomolecules in question are
specifically bound to the capture component immobilized on the fiber
core surface, the fluorophores labeled to the target biomolecules
become attached within the evanescent zone of the core surface at the
same time. Excitation of fluorophore in the evanescent zone occurs
when light is launched into the fiber. Fluorescent emission at a
longer wavelength is trapped in the fiber and is then coupled back
into the fiber. The fluorescent flux relates to the concentration of
target biomolecules.
This type of biosensors has several advantages over other fluorescence-based sensors. The evanescent wave penetrates essentially only a fraction of a wavelength into the sample medium and thus excites fluorescence primarily from the fluorescently labeled analyte complexes that have been bound to the surface through specific antibody-antigen recognition reaction (i.e., immunoassay) and hybridization of nucleic acid. Not only does this reduce the fluorescence from the sample or unbound fluorescent reagents, but the signal obtained is directly related to the binding kinetics of the detection interaction, i.e., a rapid real-time response can be observed without complicated washing or separation steps, and field detection of a variety of analytes can be performed.
Features
|
Model |
Performances |
|
FOB-2 |
l 5 fiber probes allow for simultaneous detection of 5 agents within one sample l Built-in fluidic system including computer-controlled peristaltic pump and valves, allows automated sucking and exhausting of sample, buffer and fluorophore-labeled secondary antibody l Built-in multi-function DAS card controls the test procedure, and processes and display and stores result data l A warming system is used to keep the sample at a constant temperature of 37°Cor45°C l Low sample volume, less than 2 ml |
|
FOB-3 |
l Single fiber probe l Built-in multi-function DAS card connects to either notebook or desktop computer l A warming system is used to keep the sample at a constant temperature of 37°Cor45°C l Rechargeable battery powered for 8 hrs continuous operation normally l Dimension (mm): 235(W)´300(D)´120(H) l Low sample volume, less than 250 ml |
Specifications
1) Light source: 635-nm laser diode to excite Cy5 fluorophore.
Other light sources are available for excitation of other fluorophores
2) Sensing element: fiber of fused silica, or injection-molded polystyrene fiber
3) High specificity and sensitivity lower than 0.01nmol/l for pure Cy5 solution
4) User-friendly and ease of use software involved
5) Real-time binding measurement can be performed
Fiber-optic Evanescent Wave Biosensor FOB-2
Fiber-optic Evanescent Wave Biosensor FOB-3
